Introduction 

Retesting is a vital part of how long-term gut health is supported through the BIOME9 supplement plan. Just like in human medicine, adjusting and optimising based on results is key to sustainable change. With over 100 individual retests now completed, BIOME9 has gathered robust evidence showing how precision microbiome support, guided by data and tailored supplements, can lead to measurable, wide-ranging improvements in dogs.

The Power of Retesting and Adjustment

Microbiome modulation isn’t a one-off fix. It’s a journey. BIOME9 recommends retesting after dogs have been on the supplement plan for 8 to 12 weeks. This gives both practitioners and owners a clear window into what’s changing and where further support might be needed.

In 101 individual retests, 83% of dogs showed significant improvements across core gut health scores and functions. These changes were not only reflected in lab data but also matched the improvements reported by owners: better digestion, calmer behaviour, fewer symptoms, and increased vitality.

Microbial Community Shifts: Diversity, Stability, and Beneficial Bacteria

Across the retests, the average increase in microbial diversity was striking. Diversity more than doubled, with an average of 167 additional bacterial species detected post-supplementation. Balance and stability—two hallmarks of a resilient microbiome—also improved by 39% on average.

Additionally, 68% of dogs saw an increase in all twelve core commensal (beneficial) bacteria. The most notable increases were seen in Megamonas, Fusobacterium, and Faecalibacterium—species associated with healthy digestion, anti-inflammatory functions, and immune regulation. This shift suggests that targeted supplementation supports broad microbial health and helps re-establish key populations that may have been lost or suppressed.

 

Digestive Function: Precision Nutrition at Work

Improving digestive efficiency is one of the core aims of microbiome modulation. After completing the supplement plan and retesting, dogs showed an average 12% improvement in digestive function. These gains weren’t just about symptom relief—they reflected better nutrient absorption and energy extraction from food:

• +6% protein digestion

• +9% carbohydrate digestion

• +11% fibre digestion

• +13% lipid digestion

• +20% increase in vitamin and mineral absorption

By optimising the existing diet rather than replacing it, the BIOME9 plan supports precision nutrition, helping each dog get more out of the food it already eats.

Beyond Digestion: Whole-Body Benefits

While gut health is the foundation, the benefits of a balanced microbiome extend across multiple systems. In 91% of retests, dogs showed improved health markers beyond digestion.

For example, breath odour (a common complaint) improved by 28%, reflecting internal metabolic improvements rather than superficial masking. Neurological health scores rose by 12%, supporting what broader data has shown: nearly one-third of dogs with gut issues also experience behavioural symptoms.

Perhaps most striking was the impact on immune-related functions. Retests showed:

• +8% improvement in coat and skin health

• +16% increase in immune and cardiovascular markers

• +17% reduction in markers of gut inflammation

• +27% improvement in joints and mobility

These findings reinforce the microbiome’s central role in systemic health and highlight the importance of personalised, science-based support.

What We’re Learning, and What’s Next

With over 100 retests and counting, the data speaks for itself. Personalised microbiome support doesn’t just shift numbers—it transforms health outcomes. As BIOME9’s database continues to grow, it’s providing even more confidence in this precision-based approach and expanding understanding of how to support different breeds, life stages, and health challenges.